WebJul 28, 2024 · (H) Closest apposition of the biocytin filled bouton and IN dendrite is shown in a single 200-nm-thin section. White outlines help distinguishing the two profiles throughout the immunolabeled images. (I) The same section was immunolabeled for vGluT1, PSD95, Munc13-1, Bassoon, and Piccolo and for AMPA receptors (panAMPAR Ab) in 3 labeling … WebAccurate 3D reconstruction of neurons is vital for applications linking anatomy and physiology. Reconstructions are typically created using Neurolucida after biocytin histology (BH). An alternative inexpensive and fast method is to use freeware such as Neuromantic to reconstruct from fluorescence imaging (FI) stacks acquired using 2-photon laser …
Improved biocytin labeling and neuronal 3D reconstruction
WebFeb 2, 2012 · In this report, we describe a reliable protocol for biocytin labeling of neuronal tissue and diaminobenzidine (DAB)-based processing of brain slices. WebOct 12, 2024 · b Representative images of neurons for the recordings in the VTA and the neuron filled with dye. Scale bar, 20 μm. ... o Responsive Mor-Ens from slice of mice treated with saline or escalating dose of morphine was filled with biocytin and stained with anti-TH antibody after recording to identify the TH + subtypes (Saline: n = 24 neurons … netgear wx610y
CRHCeA→VTA inputs inhibit the positive ensembles to induce …
WebIn more details, a biocytin (C 16 H 28 N 4 O 4 S) self-assembled monolayer is adsorbed onto the base of a CaF 2 prism closing a Teflon cell. The latter is then filled with a HEPES buffer solution containing test proteins: avidin, avidin presaturated with biotin (PSA), or bovine serum albumin (BSA). After 16 h of contact with the biocytin film ... WebJun 13, 2007 · To measure the length of neuronal dendrite covered by an astrocyte, confocal z-series containing the biocytin-filled neuron and EGFP-positive astrocytes were studied using Volocity software. Single astrocytes were identified as mentioned previously. Of the nine layer 2/3 neurons that were successfully loaded with and stained for … WebSep 9, 2024 · To reconstruct the full morphology of biocytin filled or GFP-labeled neurons , imaging was performed on a Leica TCS SP8 confocal laser-scanning microscope (Bensheim, Germany) using a 40x/1.3NA oil-immersion objective. Z-stack tile scans of the whole culture slice were acquired. The Cy3 fluorescence was excited at 548 nm using a … netgear x6 r8000 slow