Bmal1 chip-seq
WebJan 2024 - Oct 202410 months. San Diego, California, United States. • Experienced project leader utilizing molecular techniques and Next … WebDysregulation of circadian rhythms associates with cardiovascular disorders. It is known that deletion of the core circadian gene Bmal1 in mice causes dilated cardiomyopathy. …
Bmal1 chip-seq
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WebSep 3, 2024 · BMAL1 ChIP-Seq of exosome-treated adipocytes showed 42,037 binding sites in the DS condition and 5538 sites in the NS condition, with a large proportion of BMAL1 targets including genes encoding for metabolic regulators. A significant and restricted miRNA exosomal signature emerged after exposure to the NS condition. … WebAug 20, 2024 · A secondary approach to verify BMAL1 targets identified with ChIP-seq, was to query for overlap of targets with genes that exhibited circadian rhythms of expression in mammary tissue or identified as BMAL1 targets in hepatic tissue. The set of genes in S2 and S3 Tables were used for this analysis.
WebChIP-Seq: Library source: genomic: Library selection: ChIP: Instrument model: Illumina NovaSeq 6000 : Description: Eluted ChIP DNA from Female mouse at timepoint ZT12 of Bmal1 under the Control protocol. Data processing: ChIP-seq was processed via the nf-core ChIP-seq pipeline v1.2.2 using the narrow peaks method. Webthat BMAL1-driven rhythmic transcription depends on the capacity of BMAL1 to rhythmically regulate a network of enhancers. Results BMAL1 DNA binding signal is largely tissue specific To determine if BMAL1 cistrome differs between tissues, we performed BMAL1 ChIP-Seq in the mouse liver, kidney and heart with three biological replicates per tissue.
WebFeb 23, 2024 · A line of mammary epithelial cells with the BMAL1 86gene knocked out (BMAL1-KO) using CRISPR-CAS was created to verify ChIP-seq findings, 87and study … WebJan 4, 2024 · BMAL1 (top) and CLOCK (bottom) ChIP-Seq signal for each of the 4 CLOCK:BMAL1 transcriptional output groups. Signal is also …
WebMar 26, 2024 · To explore this possibility, we performed chromatin immuno-precipitation sequencing (ChIP-seq) experiments with specific antibodies against BMAL1 and MAX proteins. This analysis revealed a large number of genomic regions bound by MAX (around 13,000 peaks), while BMAL1 binding was limited to about 800 regions ( Figure 3 A; …
WebChIP-Seq: Library source: genomic: Library selection: ChIP: Instrument model: Illumina NovaSeq 6000 : Description: Eluted ChIP DNA from Female mouse at timepoint ZT0 of Bmal1 under the Control protocol. Data processing: ChIP-seq was processed via the nf-core ChIP-seq pipeline v1.2.2 using the narrow peaks method. todaypayme.comPreviously we discovered an extensive genome-wide colocalization of HNF4A and BMAL1::CLOCK in the mouse liver24. While physical interactions and genome-wide co-occupancy between the diurnal regulatory machinery and tissue-specific transcription factors have been reported25,26,27, to our … See more The cooperative loading of transcription factors may involve two mechanisms: (1) a simultaneous loading mediated by protein–protein interactions; (2) a sequential loading … See more The chromatin remodeling activity and rhythmic recruitment24 of HNF4A prompted us to test whether the hepatic chromatin landscape is dynamically shaped throughout … See more We previously showed that Hnf4a knockdown caused varying degrees of circadian rhythm disruption in cell cultures including period shortening and complete arrhythmicity24. Consistently, tissue explants of HKO liver … See more To evaluate how HNF4A activity is supervised by the circadian clock, we first induced chronic circadian disruption in mice by performing a jet lag protocol for 4 weeks. At the end of the treatment, remarkably, night … See more today pathan movie collectionWebAug 20, 2024 · Validation of BMAL1 antibody and quality of ChIP-seq data. To test the hypothesis that BMAL1 transcriptional targets changed with state of mammary epithelial … pension attorneys njWebChIP-Seq: Library source: genomic: Library selection: ChIP: Instrument model: Illumina NovaSeq 6000 : Description: Eluted ChIP DNA from Female mouse at timepoint ZT12 of Bmal1 under the Misalignment protocol. Data processing: ChIP-seq was processed via the nf-core ChIP-seq pipeline v1.2.2 using the narrow peaks method. pensionat torpaWebSample 1_BMAL1 ChIP-seq: GSM982689: Sample 2_BMAL1 ChIP-seq: GSM982690: Sample 3_BMAL1 ChIP-seq This SubSeries is part of SuperSeries: GSE39860: Transcriptional Architecture and Chromatin Landscape of the Core Circadian Clock in Mammals: Relations: BioProject: PRJNA172197: SRA: SRP014752: Download family: … today patio furnitureWebApr 15, 2014 · ChIP-Seq Identifies a Novel Clock Gene Regulated by BMAL1 Our previous ChIP-based genome-wide analyses using a core clock transcription factor BMAL1 identified hundreds of target molecules [22] . Among these targets, Gm129 , now called Chrono , was one of the groups with the strongest binding including core clock proteins PER and CRY. today pastry recipeWebJun 26, 2024 · Using cell fractionation and mass spectrometry we reveal that BMAL1 associates with NOP58, a core component of the box C/D small nucleolar … pensionat warfsholm gotland